Cell Line Authentication Database

Cosmic the catalogue of somatic mutations in cancer is the world s largest and most comprehensive resource for exploring the impact of somatic mutations in human cancer.

Cell line authentication database.

Among the important resources is the database of cross contaminated or misidentified cell lines 6 maintained by the international cell line authentication committee iclac a group founded in. The cells must be checked the condition whether more than one cell line is handled in a laboratory to avoid cross contamination. The register currently lists 552 cell lines. The cell line integrated molecular authentication clima database is the result of an integration effort aimed at including all certified str profiles of human cell lines in a unique database.

Cell lines project v92 released 27 aug 20. An effort has been made to exclude cell lines that legitimately come from the same donor for example if one cell line was intentionally derived from another. Authentication of a cell line is the sum of the process by which a line s identity is verified and shown to be free of contamination from other cell lines and microbes. Cell line authentication is increasingly necessary with the wide use of cell lines in research.

Using standardized techniques authentication enables communication among all users about the resource and ensures valid reproducible experimental results. Browse our resources to explore the current best practices for validating storing and handling biological materials and how these techniques support data integrity and reproducibility. Its main feature is cell line identification according to the standard ansi atcc asn 0002 2011 but it also provides features for searching profiles by. Please ask your labcorp representative for more information on the calculations labcorp uses for comparing human.

Online reference databases cell line str profiles. Consequently data derived from misidentified cell lines can be misleading or false and cause confusion in the scientific literature. Please be aware that each online cell line reference database uses their own calculations to determine the percentage match. Cell line authentication is required whenever a new cell line is established.

Human cultured cells are at risk for contamination and misidentification. To address this issue a growing number of journals and funding agencies now require researchers to authenticate their cell lines using an accepted consensus method such as short tandem repeat str profiling. It should also be conducted before freezing at any time when the line is inconsistent. Both incorrectly identified animal and human lines have been distributed repeatedly thus invalidating years of hard work.