Cellulose Acetate Paper Electrophoresis

The results were analyzed according to manufacturer s guidelines.

Cellulose acetate paper electrophoresis.

After this immerse both the ends of cellulose acetate filter paper in a buffer solution. Electrophoresis is typically performed at ph 8 6 using cellulose acetate as the support medium. 12x2 5 cm sample applied from micropipette or capillary along a line about 1 cm. Cellulose acetate electrophoresis is more or less very similar to the paper electrophoresis.

Cellulose acetate is the acetate ester of cellulose it was first prepared in 1865. On cathode side of midline current 0 4 ma cm width of paper. Widely used in analysis of clinical and biological protein sample albumin and globulins. Cellulose acetate electrophoresis 23.

Here we describe a modified version of cellulose acetate membrane electrophoresis for hemoglobin separation from blood sample. The cellulose acetate system is the ideal tank for both standard and wet cellulose acetate electrophoresis. Provides a good background or staining glycoprotein application. It involves the following steps.

The electrophoresis on cellulose acetate membrane is most widely used because of its simplicity and is without the use of any sophisticated instrument other than electrophoresis apparatus and the cellulose acetate strip. It is also used as a synthetic fiber in the manufacture of cigarette filters and playing cards in photographic film cellulose acetate replaced nitrate film in the 1950s being far. It gives sharper bands. Cellulose acetate electrophoresis it contains 2 3 acetyl groups per glucose unit and its adsorption capacity is less than that of paper.

Alternative to paper electrophoresis. Cellulose acetate is used as a film base in photography as a component in some coatings and as a frame material for eyeglasses. First the cellulose acetate filter paper moistens with the buffer solution. At this ph the overall hemoglobin molecule is negatively charged and when placed in an electric field will move towards the positive terminal anode.

It is a modified version of paper electrophore sis developed by kohn in 1958. Cellulose acetate electrophoresis is an important technique in clinical diagnostics. After staining strips are made transparent by soaking in whitmore s oil or liquid paraffin and then suitable for transmittance densitometry of the protein bands. In this type of electrophoresis bacteriological acetate mem brane filters are taken in place of regular chro matography paper.